XACT noncoding RNA competes with XIST in the control of X chromosome activity during human early development - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Cell Stem Cell Année : 2017

XACT noncoding RNA competes with XIST in the control of X chromosome activity during human early development

Résumé

Sex chromosome dosage compensation is essential in most metazoans, but the developmental timing and underlying mechanisms vary significantly, even among placental mammals. Here we identify human-specific mechanisms regulating X chromosome activity in early embryonic development. Single-cell RNA sequencing and imaging revealed co-activation and accumulation of the long noncoding RNAs (lncRNAs) XACT and XIST on active X chromosomes in both early human pre-implantation embryos and naive human embryonic stem cells. In these contexts, the XIST RNA adopts an unusual, highly dispersed organization, which may explain why it does not trigger X chromosome inactivation at this stage. Functional studies in transgenic mouse cells show that XACT influences XIST accumulation in cis. Our findings therefore suggest a mechanism involving antagonistic activity of XIST and XACT in controlling X chromosome activity in early human embryos, and they highlight the contribution of rapidly evolving lncRNAs to species-specific developmental mechanisms.
Fichier principal
Vignette du fichier
2017_Valot_CellStemCell_1.pdf (4.46 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01607750 , version 1 (26-05-2020)

Licence

Paternité

Identifiants

Citer

Céline Vallot, Catherine Patrat, Amanda J. Collier, Christophe Huret, Miguel Casanova, et al.. XACT noncoding RNA competes with XIST in the control of X chromosome activity during human early development. Cell Stem Cell, 2017, 20 (1), pp.102-111. ⟨10.1016/j.stem.2016.10.014⟩. ⟨hal-01607750⟩
324 Consultations
35 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More