Time-Specific Effects of Spindle Positioning on Embryonic Progenitor Pool Composition and Adult Neural Stem Cell Seeding - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Neuron Année : 2017

Time-Specific Effects of Spindle Positioning on Embryonic Progenitor Pool Composition and Adult Neural Stem Cell Seeding

Résumé

The developmental mechanisms regulating the number of adult neural stem cells (aNSCs) are largely unknown. Here we show that the cleavage plane orientation in murine embryonic radial glia cells (RGCs) regulates the number of aNSCs in the lateral ganglionic eminence (LGE). Randomizing spindle orientation in RGCs by overexpression of Insc or a dominant-negative form of Lgn (dnLgn) reduces the frequency of self-renewing asymmetric divisions while favoring symmetric divisions generating two SNPs. Importantly, these changes during embryonic development result in reduced seeding of aNSCs. Interestingly, no effects on aNSC numbers were observed when Insc was overexpressed in postnatal RGCs or aNSCs. These data suggest a new mechanism for controlling aNSC numbers and show that the role of spindle orientation during brain development is highly time and region dependent.

Dates et versions

hal-01691954 , version 1 (24-01-2018)

Identifiants

Citer

Sven Falk, Stéphane Bugeon, Jovica Ninkovic, Gregor-Alexander Pilz, Maria Pia Postiglione, et al.. Time-Specific Effects of Spindle Positioning on Embryonic Progenitor Pool Composition and Adult Neural Stem Cell Seeding. Neuron, 2017, 93 (4), pp.777 - 791.e3. ⟨10.1016/j.neuron.2017.02.009⟩. ⟨hal-01691954⟩
126 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More