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Article Dans Une Revue RSC Advances Année : 2017

Folate-modified silicon carbide nanoparticles as multiphoton imaging nanoprobes for cancer-cell-specific labeling

Résumé

Interest in multiphoton microscopy for cell imaging has considerably increased over the last decade. Silicon carbide (SiC) nanoparticles exhibit strong second-harmonic generation (SHG) signal, and can thus be used as nonlinear optical probes for cell imaging. In this study, the surface of SiC nanoparticles was chemically modified to enable cancer-cell-specific labeling. In a first step, an aminosilane was grafted onto the surfaceof SiC nanoparticles. The resulting nanoparticles were further modified with folic acid, using an isothiocyanate-based coupling method. Nanoparticles from different functionalization steps were investigated by zeta potential measurement, colorimetric titration, infrared and ultraviolet-visible (UV-Vis) absorption spectroscopy, X-ray photoelectron spectroscopy (XPS), and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Characterization results confirmed successful covalent grafting of silane and folic acid to nanoparticle surface. Finally, the efficacy of these folate-modified SiC nanoparticles for cancer-cell-specific labeling was evaluated by multiphoton microscopy, by measuring SHG-emitting cell area on multiphoton images. The average cancer-cell labeling percentage was about 48%, significantly higher than for negative controls (healthy cells, competition assay and poly(ethylene glycol) modified-SiC nanoparticles), where it ranged between 10% and 15%. These results demonstrated good efficiency and specificity for these folate-modified SiC nanoparticles in cancer-cell-specific labeling.

Dates et versions

hal-01701507 , version 1 (05-02-2018)

Identifiants

Citer

Maxime Boksebeld, Vasyl Kilin, Alain Geloen, Giacomo Ceccone, Ali Jaffal, et al.. Folate-modified silicon carbide nanoparticles as multiphoton imaging nanoprobes for cancer-cell-specific labeling. RSC Advances, 2017, 7 (44), pp.27361-27369. ⟨10.1039/c7ra03961a⟩. ⟨hal-01701507⟩
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