Permeability characteristics of cell-membrane pores induced by ostreolysin A/pleurotolysin B, binary pore-forming proteins from the oyster mushroom - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 2014

Permeability characteristics of cell-membrane pores induced by ostreolysin A/pleurotolysin B, binary pore-forming proteins from the oyster mushroom

Résumé

Proteins from the oyster mushroom, 15 kDa ostreolysin A (OlyA), and 59 kDa pleurotolysin B (PlyB) with a membrane attack complex/perforin (MACPF) domain, damage cell membranes as a binary cytolytic pore-forming complex. Measurements of single-channel conductance and transmembrane macroscopic current reveal that OlyA/PlyB form non-selective ion-conducting pores with broad, skewed conductance distributions in N18 neuroblastoma and CHO-K1 cell membranes. Polyethylene-glycol 8000 (hydrodynamic radius of 3.78 nm) provides almost complete osmotic protection against haemolysis, which strongly suggests a colloid-osmotic type of erythrocyte lysis. Our data indicate that OlyA/PlyB form transmembrane pores of varied sizes, as other pore-forming proteins with a MACPF domain.

Dates et versions

hal-01704407 , version 1 (08-02-2018)

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Citer

Sébastien Schlumberger, Katarina Črnigoj Kristan, Katja Ota, Robert Frangež, Jordi Molgό, et al.. Permeability characteristics of cell-membrane pores induced by ostreolysin A/pleurotolysin B, binary pore-forming proteins from the oyster mushroom. FEBS Letters, 2014, 588 (1), pp.35 - 40. ⟨10.1016/j.febslet.2013.10.038⟩. ⟨hal-01704407⟩

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