Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Acta crystallographica Section F : Structural biology communications [2014-...] Année : 2017

Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis

Résumé

PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement. Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in the structure-determination process of the periplasmic domain of PorM.
Fichier principal
Vignette du fichier
duhoo2017.pdf (968.67 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01802819 , version 1 (08-06-2018)

Identifiants

Citer

Yoan Duhoo, Jennifer Roche, Thi Trang Nhung Trinh, Aline Desmyter, Anaïs Gaubert, et al.. Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis. Acta crystallographica Section F : Structural biology communications [2014-..], 2017, 73 (5), pp.286 - 293. ⟨10.1107/S2053230X17005969⟩. ⟨hal-01802819⟩
541 Consultations
361 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More