Differential protein expression in the estuarine copepod Eurytemora affinis after diuron and alkylphenol exposures - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Environmental Toxicology and Chemistry Année : 2016

Differential protein expression in the estuarine copepod Eurytemora affinis after diuron and alkylphenol exposures

Résumé

Proteomics was used in the calanoid copepod Eurytemora affinis for screening of protein expression modifications induced by organic contaminants. The copepods were exposed in a continuous flow-through system for 86 h to environmentally relevant concentrations of contaminants representative of the pollution in the Seine Estuary (Haute-Normandie, France; diuron, 500 ng L−1; alkylphenol mixture, 1000 ng L−1). Proteome analysis of whole-body copepod extracts by 2-dimensional gel electrophoresis revealed that the contaminants induced modifications in protein expression, with the highest quantitative variations occurring after diuron exposure. Specifically, 88 and 41 proteins were differentially expressed after diuron and alkylphenol treatments, respectively. After mass spectrometry analysis, 51 (diuron exposure) and 15 (alkylphenol exposure) proteins were identified. The identified proteins were potentially related to energy metabolism, cell growth, nervous signal conductivity, excitotoxicity, oxidative stress response, and antioxidant defense. The data suggest a massive general disturbance of physiological functions of E. affinis after diuron exposure, whereas alkylphenols induced an alteration of a few targeted physiological functions. The protein expression signatures identified after contaminant exposure deserve further investigation in terms of the development of novel potential biomarkers for water quality assessment
Fichier principal
Vignette du fichier
BOULANGE-LECOMTE-Differential protein expression of the estuarine copepod.pdf (801.58 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

hal-01818605 , version 1 (14-06-2023)

Identifiants

Citer

Céline Boulange-Lecomte, Béatrice Rocher, Kevin Cailleaud, Pascal Cosette, Eléna Legrand, et al.. Differential protein expression in the estuarine copepod Eurytemora affinis after diuron and alkylphenol exposures. Environmental Toxicology and Chemistry, 2016, 35 (7), pp.1860 - 1871. ⟨10.1002/etc.3343⟩. ⟨hal-01818605⟩
92 Consultations
13 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More