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Article Dans Une Revue Nucleic Acids Research Année : 2018

Time-lapse imaging of molecular evolution by high-throughput sequencing

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High-throughput sequencing of in vitro selection could artificially provide large quantities of relic sequences from known times of molecular evolution. Here, we demonstrate how it can be used to reconstruct an empirical genealogical evolutionary (EGE) tree of an aptamer family. In contrast to classical phy-logenetic trees, this tree-diagram represents proliferation and extinction of sequences within a population during rounds of selection. Such information, which corresponds to their evolutionary fitness, is used to infer which sequences may have been mutated through the selection process that led to the appearance and spreading of new sequences. This approach was validated by the re-analysis of an in vitro selection that had previously identified an ap-tamer against Annexin A2. It revealed that this ap-tamer might be the descendant of a sequence that was more highly amplified in early rounds. It also succeeded in predicting improved variants of this ap-tamer and providing a means to understand the influence of selection pressure on evolution. This is the first demonstration that HTS can provide time-lapse imaging of the evolutionary pathway that is taken by a macromolecule during in vitro selection to evolve by successive mutations through better fitness.
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hal-01880062 , version 1 (24-09-2018)

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Nam Nguyen quang, Clément Bouvier, Adrien Henriques, Benoît Lelandais, Frederic Duconge. Time-lapse imaging of molecular evolution by high-throughput sequencing. Nucleic Acids Research, 2018, 46 (15), pp.7480-7494. ⟨10.1093/nar/gky583⟩. ⟨hal-01880062⟩
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