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Article Dans Une Revue Journal of Infectious Diseases Année : 2015

Characterization of a Novel Neutralizing Monoclonal Antibody Against Ebola Virus GP

Résumé

Ebola virus is the etiological agent of a severe hemorrhagic fever with a high mortality rate. As the only protein exposed on the surface of viral particles, the spike glycoprotein GP is the unique target for neutralizing monoclonal antibodies. In this study, we demonstrate the strong neutralization capacity of the monoclonal antibody #3327 and characterize its activity. GP residues that are required for recognition and neutralization were found to be located both in the internal fusion loop and in the receptor-binding domain. Analysis of Ebola virus entry in the presence of #3327 allows us to hypothesize that this antibody binds to the virus particle before internalization and endosomal processing of GP and likely prevents the final viral fusion step. Importantly, #3327 is able to block entry of virions bearing GP that contain the Q508 escape mutation common to a number of virus-neutralizing antibodies, and therefore provides future perspectives for treatment strategies against Ebola virus infection.

Dates et versions

hal-01909016 , version 1 (30-10-2018)

Identifiants

Citer

Olivier Reynard, Viktor E. Volchkov. Characterization of a Novel Neutralizing Monoclonal Antibody Against Ebola Virus GP. Journal of Infectious Diseases, 2015, 212 Suppl 2, pp.S372-378. ⟨10.1093/infdis/jiv303⟩. ⟨hal-01909016⟩
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