Whole blood microRNAs sequencing profiling for vasospasm in patients with aneurysmal subarachnoid hemorrhage
Résumé
Background and Purpose : Arterial vasospasm is a well-known delayed complication of aneurysmal subarachnoid hemorrhage (aSAH). However, no validated biomarker exists to help clinicians discriminating patients with aSAH who will develop vasospasm (VSP+) and identifying those who then deserve aggressive preventive therapy. We hypothesized that whole-blood miRNAs could be a source of candidate biomarkers for vasospasm.
Methods : Using a next-generation sequencing approach, we performed whole-blood miRNA profiling between VSP+patients with aSAH and patients who did not develop vasospasm (VSP−) in a prospective cohort of 32 patients. Profiling was performed on the admission day and 3 days before vasospasm.
Results : Four hundred forty-two miRNAs were highly expressed in whole blood of patients with aSAH. Among them, hsa-miR-3177-3p demonstrated significant (P=5.9×10−5; PBonferronicorrected=0.03) lower levels in VSP− compared with VSP+ patients. Looking for whole-blood mRNA correlates of hsa-miR-3177-3p, we observed some evidence that the decrease in hsa-miR-3177-3p levels after aSAH was associated with an increase in LDHA mRNA levels in VSP− (P<10–3) but not in VSP+ (P=0.66) patients.
Conclusions : Whole-blood miRNA levels of hsa-miR-3177-3p could serve as a biomarker for vasospasm.
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