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Article Dans Une Revue Stroke Année : 2018

Whole blood microRNAs sequencing profiling for vasospasm in patients with aneurysmal subarachnoid hemorrhage

Frédéric Clarençon
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Eimad Shotar

Résumé

Background and Purpose : Arterial vasospasm is a well-known delayed complication of aneurysmal subarachnoid hemorrhage (aSAH). However, no validated biomarker exists to help clinicians discriminating patients with aSAH who will develop vasospasm (VSP+) and identifying those who then deserve aggressive preventive therapy. We hypothesized that whole-blood miRNAs could be a source of candidate biomarkers for vasospasm. Methods : Using a next-generation sequencing approach, we performed whole-blood miRNA profiling between VSP+patients with aSAH and patients who did not develop vasospasm (VSP−) in a prospective cohort of 32 patients. Profiling was performed on the admission day and 3 days before vasospasm. Results : Four hundred forty-two miRNAs were highly expressed in whole blood of patients with aSAH. Among them, hsa-miR-3177-3p demonstrated significant (P=5.9×10−5; PBonferronicorrected=0.03) lower levels in VSP− compared with VSP+ patients. Looking for whole-blood mRNA correlates of hsa-miR-3177-3p, we observed some evidence that the decrease in hsa-miR-3177-3p levels after aSAH was associated with an increase in LDHA mRNA levels in VSP− (P<10–3) but not in VSP+ (P=0.66) patients. Conclusions : Whole-blood miRNA levels of hsa-miR-3177-3p could serve as a biomarker for vasospasm.
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Dates et versions

hal-01912720 , version 1 (05-11-2018)

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Anne-Sophie Pulcrano-Nicolas, Carole Proust, Frédéric Clarençon, Alice Jacquens, Claire Perret, et al.. Whole blood microRNAs sequencing profiling for vasospasm in patients with aneurysmal subarachnoid hemorrhage. Stroke, 2018, 49 (9), pp.2220 - 2223. ⟨10.1161/STROKEAHA.118.021101⟩. ⟨hal-01912720⟩
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