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Article Dans Une Revue PLoS ONE Année : 2014

The proinflammatory RAGE/NF-\kappaB pathway is involved in neuronal damage and reactive gliosis in a model of sleep apnea by intermittent hypoxia

Résumé

Sleep apnea (SA) causes long-lasting changes in neuronal circuitry, which persist even in patients successfully treated for the acute effects of the disease. Evidence obtained from the intermittent hypoxia (IH) experimental model of SA has shown neuronal death, impairment in learning and memory and reactive gliosis that may account for cognitive and structural alterations observed in human patients. However, little is known about the mechanism controlling these deleterious effects that may be useful as therapeutic targets in SA. The Receptor for Advanced Glycation End products (RAGE) and its downstream effector Nuclear Factor Kappa B (NF-\kappaB) have been related to neuronal death and astroglial conversion to the pro-inflammatory neurodegenerative phenotype. RAGE expression and its ligand S100B were shown to be increased in experimental models of SA. We here used dissociated mixed hippocampal cell cultures and male Wistar rats exposed to IH cycles and observed that NF-\kappaB is activated in glial cells and neurons after IH. To disclose the relative contribution of the S100B/RAGE/NF-\kappaB pathway to neuronal damage and reactive gliosis after IH we performed sequential loss of function studies using RAGE or S100B neutralizing antibodies, a herpes simplex virus (HSV)-derived amplicon vector that induces the expression of RAGEΔcyto (dominant negative RAGE) and a chemical blocker of NF-\kappaB. Our results show that NF-\kappaB activation peaks 3 days after IH exposure, and that RAGE or NF-\kappaB blockage during this critical period significantly improves neuronal survival and reduces reactive gliosis. Both in vitro and in vivo, S100B blockage altered reactive gliosis but did not have significant effects on neuronal survival. We conclude that both RAGE and downstream NF-\kappaB signaling are centrally involved in the neuronal alterations found in SA models, and that blockage of these pathways is a tempting strategy for preventing neuronal degeneration and reactive gliosis in SA.

Dates et versions

hal-01917854 , version 1 (09-11-2018)

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Citer

Maria Florencia Angelo, Alejandra Aguirre, Rolando X. Avilés Reyes, Alejandro Villarreal, Jerónimo Lukin, et al.. The proinflammatory RAGE/NF-\kappaB pathway is involved in neuronal damage and reactive gliosis in a model of sleep apnea by intermittent hypoxia. PLoS ONE, 2014, 9 (9), pp.e107901. ⟨10.1371/journal.pone.0107901⟩. ⟨hal-01917854⟩
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