A Novel Fluorescent Probe That Is Brain Permeable and Selectively Binds to Myelin - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Histochemistry and Cytochemistry Année : 2006

A Novel Fluorescent Probe That Is Brain Permeable and Selectively Binds to Myelin

Donghua Tian
  • Fonction : Auteur
Yue Feng
  • Fonction : Auteur
Paul Polak
  • Fonction : Auteur
Jingjun Wei
  • Fonction : Auteur
Adam Sharp
  • Fonction : Auteur
Bruno Stankoff
Bernard Zalc
  • Fonction : Auteur
  • PersonId : 855357
Elliott Mufson
  • Fonction : Auteur
Robert Gould
  • Fonction : Auteur
Douglas L. Feinstein
  • Fonction : Auteur
Yanming Wang
  • Fonction : Auteur

Résumé

Myelin is a multilayered glial cell membrane that forms segmented sheaths around large-caliber axons of both the central nervous system (CNS) and peripheral nervous system (PNS). Myelin covering insures rapid and efficient transmission of nerve impulses. Direct visual assessment of local changes of myelin content in vivo could greatly facilitate diagnosis and therapeutic treatments of myelin-related diseases. Current histologic probes for the visualization of myelin are based on antibodies or charged histochemical reagents that do not enter the brain. We have developed a series of chemical compounds including (E,E)-1,4-bis(4'-aminostyryl)-2-dimethoxy-benzene termed BDB and the subject of this report, which readily penetrates the blood-brain barrier and selectively binds to the myelin sheath in brain. BDB selectively stains intact myelinated regions in wild-type mouse brain, which allows for delineation of cuprizone-induced demyelinating lesions in mouse brain. BDB can be injected IV into the brain and selectively detect demyelinating lesions in cuprizone-treated mice in situ. These studies justified further investigation of BDB as a potential myelin-imaging probe to monitor myelin pathology in vivo.

Dates et versions

hal-01936287 , version 1 (27-11-2018)

Identifiants

Citer

Chunying Wu, Donghua Tian, Yue Feng, Paul Polak, Jingjun Wei, et al.. A Novel Fluorescent Probe That Is Brain Permeable and Selectively Binds to Myelin. Journal of Histochemistry and Cytochemistry, 2006, 54 (9), pp.997 - 1004. ⟨10.1369/jhc.5A6901.2006⟩. ⟨hal-01936287⟩
22 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More