Rearrangements under confinement lead to increased binding energy of Synaptotagmin-1 with anionic membranes in Mg 2+ and Ca 2+ - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 2018

Rearrangements under confinement lead to increased binding energy of Synaptotagmin-1 with anionic membranes in Mg 2+ and Ca 2+

Résumé

Synaptotagmin‐1 (Syt1) is the primary calcium sensor (Ca2+) that mediates neurotransmitter release at the synapse. The tandem C2 domains (C2A and C2B) of Syt1 exhibit functionally critical, Ca2+‐dependent interactions with the plasma membrane. With the surface forces apparatus, we directly measure the binding energy of membrane‐anchored Syt1 to an anionic membrane and find that Syt1 binds with ~6 kBT in EGTA, ~10 kBT in Mg2+ and ~18 kBT in Ca2+. Molecular rearrangements measured during confinement are more prevalent in Ca2+ and Mg2+ and suggest that Syt1 initially binds through C2B, then reorients the C2 domains into the preferred binding configuration. These results provide energetic and mechanistic details of the Syt1 Ca2+‐activation process in synaptic transmission.
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Dates et versions

hal-01954084 , version 1 (13-12-2018)

Identifiants

Citer

Clémence Gruget, Jeff Coleman, Oscar D Bello, Shyam S Krishnakumar, Eric Perez, et al.. Rearrangements under confinement lead to increased binding energy of Synaptotagmin-1 with anionic membranes in Mg 2+ and Ca 2+. FEBS Letters, 2018, 592 (9), pp.1497-1506. ⟨10.1002/1873-3468.13040⟩. ⟨hal-01954084⟩
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