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Article Dans Une Revue PLoS ONE Année : 2013

Selective Individual Primary Cell Capture Using Locally Bio-Functionalized Micropores

Résumé

Background Solid-state micropores have been widely employed for 6 decades to recognize and size flowing unlabeled cells. However, the resistive-pulse technique presents limitations when the cells to be differentiated have overlapping dimension ranges such as B and T lymphocytes. An alternative approach would be to specifically capture cells by solid-state micropores. Here, the inner wall of 15-µm pores made in 10 µm-thick silicon membranes was covered with antibodies specific to cell surface proteins of B or T lymphocytes. The selective trapping of individual unlabeled cells in a bio-functionalized micropore makes them recognizable just using optical microscopy. Methodology/Principal Findings We locally deposited oligodeoxynucleotide (ODN) and ODN-conjugated antibody probes on the inner wall of the micropores by forming thin films of polypyrrole-ODN copolymers using contactless electro-functionalization. The trapping capabilities of the bio-functionalized micropores were validated using optical microscopy and the resistive-pulse technique by selectively capturing polystyrene microbeads coated with complementary ODN. B or T lymphocytes from a mouse splenocyte suspension were specifically immobilized on micropore walls functionalized with complementary ODN-conjugated antibodies targeting cell surface proteins. Conclusions/Significance The results showed that locally bio-functionalized micropores can isolate target cells from a suspension during their translocation throughout the pore, including among cells of similar dimensions in complex mixtures.

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hal-01983220 , version 1 (16-01-2019)

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Jie Liu, Radoslaw Bombera, Loïc Leroy, Yoann Roupioz, Dieu-Donné Baganizi, et al.. Selective Individual Primary Cell Capture Using Locally Bio-Functionalized Micropores. PLoS ONE, 2013, 8 (3), pp.e57717. ⟨10.1371/journal.pone.0057717⟩. ⟨hal-01983220⟩
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