Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant oncentrations - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Frontiers in Environmental Science Année : 2019

Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant oncentrations

Résumé

The impact of a microplastic (MP) mixture composed of polyethylene (PE) andpolypropylene (PP) plastic particles, prepared from commercially available products,was evaluated in blue mussels Mytilus spp. exposed to three environmentally relevantconcentrations: 0.008 μg L−1 (low), 10 μg L−1 (medium), and 100 μg L−1 (high).Organisms were exposed for 10 days followed by 10 days of depuration in clean seawaterunder controlled laboratory conditions. The evaluation of MP effects on mussel clearancerate, tissue structure, antioxidant defenses, immune and digestive parameters, and DNAintegrity were investigated while the identification of plastic particles in mussel tissues(gills, digestive gland, and remaining tissues), and biodeposits (feces and pseudofaeces)was performed using infrared microscopy (μFT-IR). Results showed the presence of MPsonly in the digestive gland of mussels exposed to the highest tested concentration ofMPs with a mean of 0.75 particle/mussel (after the 10 days of exposure). In biodeposits,PE and PP particles were detected following exposure to all tested concentrationsconfirming the ingestion of MPs by the organisms. A differential response of antioxidantenzyme activities between digestive gland and gills was observed. Significant increasesin superoxide dismutase (SOD) and catalase (CAT) activities were measured in thedigestive gland of mussels exposed to the low (0.008 μg L−1) and medium (10 μg L−1)concentrations ofMPs and in the gills frommussels exposed to the highest concentration(100 μg L−1) of MPs that could be indicative of a change in the redox balance. Moreover,an increase in acid phosphatase activity was measured in hemolymph of musselsexposed to 0.008 and 10 μg L−1concentrations. No significant difference was observedin the clearance rate, and histopathological parameters between control and exposedmussels. This study brings new insights on the potential sublethal impacts of MPs atenvironmentally relevant concentrations in marine bivalves.
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Dates et versions

hal-02146660 , version 1 (16-07-2020)

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Messika Revel, Fabienne Lagarde, Hanane Perrein-Ettajani, Mélanie Bruneau, Farida Akcha, et al.. Tissue-Specific Biomarker Responses in the Blue Mussel Mytilus spp. Exposed to a Mixture of Microplastics at Environmentally Relevant oncentrations. Frontiers in Environmental Science, 2019, ⟨10.3389/fenvs.2019.00033⟩. ⟨hal-02146660⟩
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