The COMA complex interacts with Cse4 and positions Sli15/Ipl1 at the budding yeast inner kinetochore - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue eLife Année : 2019

The COMA complex interacts with Cse4 and positions Sli15/Ipl1 at the budding yeast inner kinetochore

Josef Fischböck-Halwachs
  • Fonction : Auteur
Sylvia Singh
  • Fonction : Auteur
Mia Potocnjak
  • Fonction : Auteur
Götz Hagemann
  • Fonction : Auteur
Victor Solis-Mezarino
  • Fonction : Auteur
Stephan Woike
  • Fonction : Auteur
Medini Ghodgaonkar Steger
  • Fonction : Auteur
Florian Weissmann
  • Fonction : Auteur
Laura D. Gallego
  • Fonction : Auteur
Julie Rojas
  • Fonction : Auteur
Franz Herzog
  • Fonction : Auteur

Résumé

Kinetochores are macromolecular protein complexes at centromeres that ensure accurate chromosome segregation by attaching chromosomes to spindle microtubules and integrating safeguard mechanisms. The inner kinetochore is assembled on CENP-A nucleosomes and has been implicated in establishing a kinetochore-associated pool of Aurora B kinase, a chromosomal passenger complex (CPC) subunit, which is essential for chromosome biorientation. By performing crosslink-guided in vitro reconstitution of budding yeast kinetochore complexes we showed that the Ame1/Okp1CENP-U/Q heterodimer, which forms the COMA complex with Ctf19/Mcm21CENP-P/O, selectively bound Cse4CENP-A nucleosomes through the Cse4 N-terminus. The Sli15/Ipl1INCENP/Aurora-B core-CPC interacted with COMA in vitro through the Ctf19 C-terminus whose deletion affects accurate chromosome segregation in a Sli15 wild-type background. Tethering Sli15 to Ame1/Okp1 rescued synthetic lethality upon Ctf19 depletion in a Sli15 centromere-targeting deficient mutant. This study shows molecular characteristics of the point-centromere inner kinetochore architecture and suggests a role for the Ctf19 C-terminus in mediating accurate chromosome segregation.

Dates et versions

hal-02164045 , version 1 (24-06-2019)

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Citer

Josef Fischböck-Halwachs, Sylvia Singh, Mia Potocnjak, Götz Hagemann, Victor Solis-Mezarino, et al.. The COMA complex interacts with Cse4 and positions Sli15/Ipl1 at the budding yeast inner kinetochore. eLife, 2019, 8, ⟨10.7554/eLife.42879⟩. ⟨hal-02164045⟩
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