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Article Dans Une Revue Virus Research Année : 2017

Insights in luteovirid structural biology guided by chemical cross-linking and high resolution mass spectrometry

Mariko Alexander
  • Fonction : Auteur
Jared Mohr
  • Fonction : Auteur
Stacy Deblasio
  • Fonction : Auteur
Juan Chavez
  • Fonction : Auteur
Veronique Brault
  • Fonction : Auteur
  • PersonId : 1050206
James Bruce
  • Fonction : Auteur
Michelle Heck
  • Fonction : Auteur

Résumé

Interactions among plant pathogenic viruses in the family Luteoviridae and their plant hosts and insect vectors are governed by the topology of the viral capsid, which is the sole vehicle for long distance movement of the viral genome. Previous application of a mass spectrometry-compatible cross-linker to preparations of the luteovirid Potato leafroll virus (PLRV; Luteoviridae: Polerovirus) revealed a detailed network of interactions between viral structural proteins and enabled generation of the first cross-linking guided coat protein models. In this study, we extended application of chemical cross-linking technology to the related Turnip yellows virus (TuYV; Luteoviridae: Polerovirus). Remarkably, all cross-links found between sites in the viral coat protein found for TuYV were also found in PLRV. Guided by these data, we present two models for the TuYV coat protein trimer, the basic structural unit of luteovirid virions. Additional cross-links found between the TuYV coat protein and a site in the viral protease domain suggest a possible role for the luteovirid protease in regulating the structural biology of these viruses.

Dates et versions

hal-02175707 , version 1 (05-07-2019)

Identifiants

Citer

Mariko Alexander, Jared Mohr, Stacy Deblasio, Juan Chavez, Veronique Graff, et al.. Insights in luteovirid structural biology guided by chemical cross-linking and high resolution mass spectrometry. Virus Research, 2017, 241, pp.42-52. ⟨10.1016/j.virusres.2017.05.005⟩. ⟨hal-02175707⟩
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