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Article Dans Une Revue Antimicrobial Agents and Chemotherapy Année : 2018

Genetic and functional characterization of an MCR-3-like producing Escherichia coli recovered from swine, Brazil

Résumé

A collection of 126 pigs were screened for carriage of colistin-resistant Enterobacteriaceae in a farm in Minas Gerais, Brazil. Out of this collection, eigth colistin-resistant Escherichia coli isolates were recovered, including one from Minas Gerais State, producing a new MCR-3 variant (MCR-3.12). Analysis of the lipopolysaccharide revealed that MCR-3.12 had a similar function as MCR-1 and MCR-2 by adding a phosphoethanolamine group to the lipid A. Genetic analysis showed that the mcr-3.12 gene was carried by an IncA/C2 plasmid and was embedded in an original genetic environment. This study reports the occurrence of the MCR-3-like determinant in South America and firstly demonstrates the functionality of this group of enzymes as a phosphoethanolamine transferase.

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Dates et versions

hal-02179878 , version 1 (11-07-2019)

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Nicolas Kieffer, Patrice Nordmann, Andrea Micke Moreno, Luisa Zanolli Moreno, Richard Chaby, et al.. Genetic and functional characterization of an MCR-3-like producing Escherichia coli recovered from swine, Brazil. Antimicrobial Agents and Chemotherapy, 2018, ⟨10.1128/AAC.00278-18⟩. ⟨hal-02179878⟩
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