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Article Dans Une Revue Phytochemistry Année : 2007

NMR-based fluxomics: Quantitative 2D NMR methods for isotopomers analysis

Résumé

We have investigated the reliability of 2D-COSY and 2D-TOCSY experiments to provide accurate measurements of (13)C-enrichments in complex mixtures of (13)C-labelled metabolites. This was done from both theoretical considerations and experimental investigations. The results showed that 2D-TOCSY but not 2D-COSY could provide accurate measurements of (13)C-enrichments, provided efficient zero-quantum filters were applied during the mixing period. This approach extends the range of NMR methods applicable in (13)C-labelling experiments and is suitable to investigating the dynamic behaviour of metabolic systems.

Dates et versions

hal-02183162 , version 1 (15-07-2019)

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Stephane Massou, Cécile Nicolas, Fabien Letisse, Jean-Charles Portais. NMR-based fluxomics: Quantitative 2D NMR methods for isotopomers analysis. Phytochemistry, 2007, 68 (16-18), pp.2330-2340. ⟨10.1016/j.phytochem.2007.03.011⟩. ⟨hal-02183162⟩
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