New Culture Media for Fast, Sensitive and User-Friendly Detection of E.Coli and/or Coliform Bacteria
Résumé
Objective: Bacterial contamination of drinking water is a public health concern ruled by national standards and by the world health organization guidelines. Efforts have thus been constantly devoted to improve detection of markers of contamination as early as possible any time contamination is suspected. In this work, we optimize detection of coliform bacteria in general, and of E. coli in particular, by modifying specific growth media.
Methods and results: By reinvestigating key parameters such as selectivity, growth kinetics, signal intensity, number of steps in sample manipulation and signal monitoring, we succeeded in i) improving the rate of bacterial contamination detection (medium SMIL), ii) finely tuning the ratio of contamination revelation over bacterial growth (medium SMIM), or iii) designing a user-friendly and cost-effective signal detection system (Chromo-SMIM medium).
Conclusions: SMIM and SMIM-derivate media present a better β-galactosidase (present in all coliforms) activity detection compared to classical methods. This new method thus is a useful improvement of standard techniques used worldwide in water-control analytical laboratories.
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...