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Article Dans Une Revue Molecular Cell Année : 2017

Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells

Hiroki Tanaka
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Yohann Coute
Takashi Urahama
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Patrick Héry
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Résumé

Histone replacement by transition proteins (TPs) and protamines (Prms) constitutes an essential step for the successful production of functional male gametes, yet nothing is known on the underlying functional interplay between histones, TPs, and Prms. Here, by studying spermatogenesis in the absence of a spermatid-specific histone variant, H2A.L.2, we discover a fundamental mechanism involved in the transformation of nucleosomes into nucleoprotamines. H2A.L.2 is synthesized at the same time as TPs and enables their loading onto the nucleosomes. TPs do not displace histones but rather drive the recruitment and processing of Prms, which are themselves responsible for histone eviction. Altogether, the incorporation of H2A.L.2 initiates and orchestrates a series of successive transitional states that ultimately shift to the fully compacted genome of the mature spermatozoa. Hence, the current view of histone-to-nucleoprotamine transition should be revisited and include an additional step with H2A.L.2 assembly prior to the action of TPs and Prms.

Dates et versions

hal-02191299 , version 1 (23-07-2019)

Identifiants

Citer

Sophie Barral, Yuichi Morozumi, Hiroki Tanaka, Emilie Montellier, Jérôme Govin, et al.. Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells. Molecular Cell, 2017, 66 (1), pp.89-101.e8. ⟨10.1016/j.molcel.2017.02.025⟩. ⟨hal-02191299⟩
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