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Article Dans Une Revue Journal of Biophotonics Année : 2018

Label-free non-linear microscopy to measure myelin outcome in a rodent model of Charcot-Marie-Tooth diseases

Résumé

Myelin sheath produced by Schwann cells covers and nurtures axons to speed up nerve conduction in peripheral nerves. Demyelinating peripheral neuropathies result from the loss of this myelin sheath and so far, no treatment exists to prevent Schwann cell demyelination. One major hurdle to design a therapy for demyelination is the lack of reliable measures to evaluate the outcome of the treatment on peripheral myelin in patients but also in living animal models. Non-linear microscopy techniques SHG, THG and CARS were used to image myelin ex vivo and in vivo in the sciatic nerve of healthy and demyelinating mice and rats. SHG did not label myelin and THG required too much light power to be compatible with live imaging. CARS is the most reliable of these techniques for in vivo imaging and it allows for the analysis and quantification of myelin defects in a rat model of CMT1A disease. This microscopic technique therefore constitutes a promising, reliable and robust readout tool in the development of new treatments for demyelinating peripheral neuropathies.
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Dates et versions

hal-02266920 , version 1 (16-08-2019)

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Helene Hajjar, Hassan Boukhaddaoui, Amel Rizgui, Chamroeun Sar, Jade Berthelot, et al.. Label-free non-linear microscopy to measure myelin outcome in a rodent model of Charcot-Marie-Tooth diseases. Journal of Biophotonics, 2018, 11 (12), pp.e201800186. ⟨10.1002/jbio.201800186⟩. ⟨hal-02266920⟩
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