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Article Dans Une Revue European Journal of Neuroscience Année : 1999

The interaction between F3 immunoglobulin domains and protein tyrosine phosphatases ζ/β triggers bidirectional signalling between neurons and glial cells

Résumé

F3, a mouse glycosyl-phosphatidylinositol anchored molecule of the immunoglobulin superfamily, is known to influence axonal growth and fasciculation via multiple interactions of its modular immunoglobulin-like domains. We prepared an Fc chimeric molecule (F3IgFc) to identify molecules interacting with these domains and characterize the functional impact of the interactions. We affinity-isolated tenascin-C and isoforms of the proteoglycan-type protein tyrosine phosphatases zeta/beta (PTPzeta/RPTPbeta) from extracts of developing mouse brain. We showed that both PTPzeta/RPTPbeta and tenascin-C can bind directly to F3, possibly in an exclusive manner, with the highest affinity for the F3-PTPzeta/RPTPbeta interaction. We observed a strong binding of F3IgFc-coated fluorospheres to astrocytes in neural primary cultures and to C6 astrocytoma cells, and demonstrated, in antibody perturbation experiments, that F3-Ig binding on astrocytes depends on its interaction with PTPzeta/RPTPbeta. We also found by confocal analysis that tenascin-C and PTPzeta/RPTPbeta were colocalized on astrocytes which suggests a complex interplay of interactions between PTPzeta/RPTPbeta, tenascin-C and F3. We showed that the interaction between PTPzeta/RPTPbeta and F3-Ig-like domains can trigger bidirectional signalling. C6 glia-expressed PTPzeta/RPTPbeta stimulated neurite outgrowth by cortical and cerebellar neurons, whereas preclustered F3IgFc specifically modified the distribution of phosphotyrosine labelling in these glial cells. Both effects could be prevented and/or mimicked by anti-F3 and anti-6B4PG antibodies. These results identify F3 and PTPzeta/RPTPbeta as potential mediators of a reciprocal exchange of information between glia and neurons.

Dates et versions

hal-02325987 , version 1 (22-10-2019)

Identifiants

Citer

Jean-Michel Revest, Catherine Faivre-Sarrailh, Nobuaki Maeda, Masaharu Noda, Melitta Schachner, et al.. The interaction between F3 immunoglobulin domains and protein tyrosine phosphatases ζ/β triggers bidirectional signalling between neurons and glial cells. European Journal of Neuroscience, 1999, 11 (4), pp.1134-1147. ⟨10.1046/j.1460-9568.1999.00521.x⟩. ⟨hal-02325987⟩

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