Pulsed cavitational therapy using high-frequency ultrasound for the treatment of deep vein thrombosis in an in vitro model of human blood clot - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Physics in Medicine and Biology Année : 2017

Pulsed cavitational therapy using high-frequency ultrasound for the treatment of deep vein thrombosis in an in vitro model of human blood clot

Résumé

Post-thrombotic syndrome, a frequent complicationof deepvenousthrombosis, can be reduced with early veinrecanalization.Pulsed cavitational therapy(PCT) using ultrasound is a recent non-invasive approach. We propose to test the efficacy and safety of high-frequency focused PCT for drug-free thrombolysis(thrombotripsy) in a realistic in vitro model ofvenousthrombosis. Toreproducevenous thrombosisconditions, humanwholebloodwasallowedtoclotby stasis in silicone (6mm internal diameter) at a 30 cm H2O pressure, maintained during the whole experiment. We engineered an utrasound d evice composied of dual 2.25 MHz transducers centered around a 6 MHz imaging probe. A therapeutic focus was generated at a 3.2 cm depth from the probe. Thrombotripsy was performed by longitudinally scanning the thrombus at three different speeds: 1 mm s-1 (n  =  6); 2 mm s-1 (n  =  6); 3 mm s-1 (n  =  12). Restored outflow was measured every three passages. Filters were placed to evaluate the debris size. Twenty-four occlusive thrombi, of 2.5 cm mean length and 4.4 kPa mean stiffness, were studied. Flow restoration was systematically obtained by nine subsequent passages (4.5 min maximum). By varying the device's speed, we found an optimal speed of 1 mm s-1 to be efficient for effective recanalization with 90 s (three passages). Within 90 s, flow restoration was of 80, 62 and 74% at respectively 1, 2 and 3 mm s-1. For all groups, cavitation cloud drilled a 1.7 mm mean diameter channel throughout the clot. Debris analysis showed 92% of debris  <10 µm, with no fragment  >  200 µm.
Fichier principal
Vignette du fichier
Goudot G 2017 accepted.pdf (5.63 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02327100 , version 1 (22-10-2019)

Identifiants

Citer

G Goudot, T Mirault, Benoît Arnal, C Boisson-Vidal, B Le Bonniec, et al.. Pulsed cavitational therapy using high-frequency ultrasound for the treatment of deep vein thrombosis in an in vitro model of human blood clot. Physics in Medicine and Biology, 2017, 62 (24), pp.9282-9294. ⟨10.1088/1361-6560/aa9506⟩. ⟨hal-02327100⟩
86 Consultations
94 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More