Photoswitchable phase separation and oligonucleotide trafficking in DNA coacervate microdroplets - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Angewandte Chemie International Edition Année : 2019

Photoswitchable phase separation and oligonucleotide trafficking in DNA coacervate microdroplets

Résumé

Coacervate micro-droplets produced by liquid-liquid phase separation have been used as synthetic protocells that mimic the dynamical organization of membrane-free organelles in living systems. Achieving spatiotemporal control over droplet condensation and disassembly remains challenging. Herein, we describe the formation and photo-switchable behaviour of light-responsive coacervate droplets prepared from mixtures of double-stranded DNA and an azobenzene cation. The droplets disassemble and reassemble under UV and blue light, respectively, due to azobenzene trans/cis photo-isomerisation. Sequestration and release of captured oligonucleotides follows the dynamics of phase separation such that light-activated transfer, mixing, hybridization and trafficking of the oligonucleotides can be controlled in binary populations of the droplets. Our results open perspectives for the spatiotemporal control of DNA coacervates and provide a step towards the dynamic regulation of synthetic protocells.
Fichier principal
Vignette du fichier
HAL_Martin_P119-12626.pdf (678.24 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02330433 , version 1 (24-10-2019)

Identifiants

Citer

Nicolas Martin, Liangfei Tian, Dan Spencer, Angélique Coutable-Pennarun, J. L. Ross Anderson, et al.. Photoswitchable phase separation and oligonucleotide trafficking in DNA coacervate microdroplets. Angewandte Chemie International Edition, 2019, 58 (41), pp.14594-14598. ⟨10.1002/anie.201909228⟩. ⟨hal-02330433⟩

Collections

CNRS INC-CNRS
67 Consultations
70 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More