Synergistic Activity of Floor-Plate- and Ventricular-Zone-Derived Netrin-1 in Spinal Cord Commissural Axon Guidance - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Neuron Année : 2019

Synergistic Activity of Floor-Plate- and Ventricular-Zone-Derived Netrin-1 in Spinal Cord Commissural Axon Guidance

Résumé

In vertebrates, commissural axons extend ventrally toward the floor plate in the spinal cord and hindbrain. Netrin-1, secreted by floor plate cells, was proposed to attract commissural axons at a distance. However, recent genetic studies in mice have shown that netrin-1 is also produced by ventricular zone (VZ) progenitors and that in the hindbrain, it represents the main source of netrin-1 for commissural axons. Here, we show that genetically deleting netrin-1 either from the VZ or the floor plate does not prevent midline crossing in the spinal cord, although axon pathfinding and fasciculation are perturbed. Strikingly, the VZ and floor plate act synergistically, as the simultaneous ablation of netrin-1 from these two sources severely impedes crossing. These results suggest that floor-plate-derived netrin-1 has a distinct impact on commissural axons in the spinal cord and hindbrain.

Domaines

Neurobiologie
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Dates et versions

hal-02340095 , version 1 (30-10-2019)

Identifiants

Citer

Juan Antonio Moreno-Bravo, Sergi Roig Puiggros, Patrick Mehlen, Alain Chédotal. Synergistic Activity of Floor-Plate- and Ventricular-Zone-Derived Netrin-1 in Spinal Cord Commissural Axon Guidance. Neuron, 2019, 101 (4), pp.625-634.e3. ⟨10.1016/j.neuron.2018.12.024⟩. ⟨hal-02340095⟩
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