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Article Dans Une Revue Scientific Reports Année : 2016

Impact of sialic acids on the molecular dynamic of bi-antennary and tri-antennary glycans

Pascal Maurice
Vincent Durlach
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Stéphanie Baud

Résumé

Sialic acids (SA) are monosaccharides that can be located at the terminal position of glycan chains on a wide range of proteins. The post-translational modifications, such as N-glycan chains, are fundamental to protein functions. Indeed, the hydrolysis of SA by specific enzymes such as neuraminidases can lead to drastic modifications of protein behavior. However, the relationship between desialylation of N-glycan chains and possible alterations of receptor function remains unexplored. Thus, the aim of the present study is to establish the impact of SA removal from N-glycan chains on their conformational behavior. We therefore undertook an in silico investigation using molecular dynamics to predict the structure of an isolated glycan chain. We performed, for the first time, 3 independent 500 ns simulations on bi-antennary and tri-antennary glycan chains displaying or lacking SA. We show that desialylation alters both the preferential conformation and the flexibility of the glycan chain. This study suggests that the behavior of glycan chains induced by presence or absence of SA may explain the changes in the protein function.
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Dates et versions

hal-02347497 , version 1 (05-11-2019)

Identifiants

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Alexandre Guillot, Manuel Dauchez, Nicolas Belloy, Jessica Jonquet, Laurent Duca, et al.. Impact of sialic acids on the molecular dynamic of bi-antennary and tri-antennary glycans. Scientific Reports, 2016, 6 (1), ⟨10.1038/srep35666⟩. ⟨hal-02347497⟩
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