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Article Dans Une Revue Scientific Reports Année : 2019

PTMselect: optimization of protein modifications discovery by mass spectrometry

Renaud Perchey
  • Fonction : Auteur
Marie Tosolini
Arnaud Besson

Résumé

Discovery of protein modification sites relies on protein digestion by proteases and mass spectrometry (MS) identification of the modified peptides. Depending on proteases used and target protein sequence, this method yields highly variable coverage of modification sites. We introduce PTMselect, a digestion-simulating software which tailors the optimal set of proteases for discovery of global or targeted modification from any single or multiple proteins.

Dates et versions

hal-02347515 , version 1 (05-11-2019)

Identifiants

Citer

Renaud Perchey, Laure Tonini, Marie Tosolini, Jean-Jacques Fournié, Frédéric Lopez, et al.. PTMselect: optimization of protein modifications discovery by mass spectrometry. Scientific Reports, 2019, 9 (1), pp.4181. ⟨10.1038/s41598-019-40873-3⟩. ⟨hal-02347515⟩
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