Kizuna is a novel mitotic substrate for CDC25B phosphatase - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Cell Cycle Année : 2015

Kizuna is a novel mitotic substrate for CDC25B phosphatase

Résumé

CDC25 dual-specificity phosphatases play a central role in cell cycle control through the activation of Cyclin-Dependent Kinases (CDKs). Expression during mitosis of a stabilized CDC25B mutant (CDC25B-DDA), which cannot interact with the F-box protein βTrCP for proteasome-dependent degradation, causes mitotic defects and chromosome segregation errors in mammalian cells. We found, using the same CDC25B mutant, that stabilization and failure to degrade CDC25B during mitosis lead to the appearance of multipolar spindle cells resulting from a fragmentation of pericentriolar material (PCM) and abolish mitotic Plk1-dependent phosphorylation of Kizuna (Kiz), which is essential for the function of Kiz in maintaining spindle pole integrity. Thus, in mitosis Kiz is a new substrate of CDC25B whose dephosphorylation following CDC25B stabilization leads to the formation of multipolar spindles. Furthermore, endogenous Kiz and CDC25B interact only in mitosis, suggesting that Kiz phosphorylation depends on a balance between CDC25B and Plk1 activities. Our data identify a novel mitotic substrate of CDC25B phosphatase that plays a key role in mitosis control.
Fichier principal
Vignette du fichier
kccy-13-24-972882.pdf (955.68 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02353356 , version 1 (05-12-2023)

Identifiants

Citer

Yann Thomas, Marion Peter, Francisca Méchali, Jean-Marie Blanchard, Olivier Coux, et al.. Kizuna is a novel mitotic substrate for CDC25B phosphatase. Cell Cycle, 2015, 13 (24), pp.3867-3877. ⟨10.4161/15384101.2014.972882⟩. ⟨hal-02353356⟩
30 Consultations
3 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More