Shared and differential features of Robo3 expression pattern in amniotes
Résumé
In Bilaterians, commissural neurons project their axons across the midline of the nervous system to target neurons on the opposite side. In mammals, midline crossing at the level of the hindbrain and spinal cord requires the Robo3 receptor which is transiently expressed by all commissural neurons. Unlike other Robo receptors, mammalian Robo3 receptors do not bind Slit ligands and promote midline crossing. Surprisingly, not much is known about Robo3 distribution and mechanism of action in other vertebrate species. Here, we have used whole-mount immunostaining, tissue clearing and light-sheet fluorescent microscopy to study Robo3 expression pattern in embryonic tissue from diverse representatives of amniotes at distinct stages, including squamate (African house snake), birds (chicken, duck, pigeon, ostrich, emu and zebra finch), early postnatal marsupial mammals (fat-tailed dunnart), and eutherian mammals (mouse and human). The analysis of this rich and unique repertoire of amniote specimens reveals conserved features of Robo3 expression in midbrain, hindbrain and spinal cord commissural circuits, which together with subtle but meaningful modifications could account for species-specific evolution of sensory-motor and cognitive capacities. Our results also highlight important differences of precerebellar nuclei development across amniotes.
Mots clés
RRID:AB_1078266
RRID:AB_2107107
RRID:AB_2107124
RRID:AB_2181865
RRID:AB_2307443
RRID:AB_2313773
RRID:AB_2315065
RRID:AB_2340880
RRID:AB_2340885
RRID:AB_2492288
RRID:AB_262133
RRID:AB_2651135
RRID:AB_2732861
RRID:AB_881306
RRID:SCR_007370
Robo3
amniotes
commissures
development
evolution
habenula
inferior olive
pontine nucleus
Domaines
Sciences du Vivant [q-bio]
Fichier principal
Friocourt et al. - 2019 - Shared and differential features of Robo3 expressi.pdf (4.61 Mo)
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Origine : Fichiers produits par l'(les) auteur(s)
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