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Article Dans Une Revue EMBO Reports Année : 2019

Article Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata

Marie L Brault
Françoise Immel
Marie Glavier
Amélia Gaston
Mathieu Fouche
Stéphane Claverol
Andrea Paterlini

Résumé

In eukaryotes, membrane contact sites (MCS) allow direct communication between organelles. Plants have evolved a unique type of MCS, inside intercellular pores, the plasmodesmata, where endoplas-mic reticulum (ER)-plasma membrane (PM) contacts coincide with regulation of cell-to-cell signalling. The molecular mechanism and function of membrane tethering within plasmodesmata remain unknown. Here, we show that the multiple C2 domains and trans-membrane region protein (MCTP) family, key regulators of cell-to-cell signalling in plants, act as ER-PM tethers specifically at plasmodes-mata. We report that MCTPs are plasmodesmata proteins that insert into the ER via their transmembrane region while their C2 domains dock to the PM through interaction with anionic phospholipids. A Atmctp3/Atmctp4 loss of function mutant induces plant developmental defects, impaired plasmodesmata function and composition, while MCTP4 expression in a yeast Dtether mutant partially restores ER-PM tethering. Our data suggest that MCTPs are unique membrane tethers controlling both ER-PM contacts and cell-to-cell signalling.
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hal-02368086 , version 1 (18-11-2019)

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Marie L Brault, Jules D Petit, Françoise Immel, William J Nicolas, Marie Glavier, et al.. Article Multiple C2 domains and transmembrane region proteins (MCTPs) tether membranes at plasmodesmata. EMBO Reports, 2019, 20 (8), pp.:e47182. ⟨10.15252/embr.201847182⟩. ⟨hal-02368086⟩
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