Nanofiltration of extracellular vesicles from human plasma & their on-chip qualification and quantification with a NanoBioAnalytical platform.
Résumé
One complication of plasma transfusion is non-immune transfusion related acute lung injury (TRALI), which is associated with the formation of neutrophil extracellular traps (NETs) resulting from polymorphonuclear neutrophil (PMN) activation. Activated platelets have recently been shown to trigger NETs formation. We hypothesized that (a) NETs could be promoted by platelet derived microparticles (PMPs) and (b) that 75 nm-nanofiltration of plasma, by removing parts of PMPs, could avoid NETs formation. To better characterize the large spectrum of size and concentration of PMPs in complex media, we combined conventional approaches (Flow Cytometry, TRPS) with an original Nanobioanalytical (NBA) platform based on Surface Plasmon Resonance imaging (SPRi) and Atomic Force Microscopy (AFM) techniques.