Methods to Determine Interaction Interfaces Between β-Arrestins and Their Protein Partners
Résumé
β-arrestins are so-called hub proteins: they make complexes with many different partners, assembling functional complexes, and thereby fulfilling their biological function. The importance of this process in G protein-coupled receptor (GPCR) signalling has been fully demonstrated for many different receptors. For direct interactions, determining the interface regions, on β-arrestins and on the partners, is crucial for understanding the function of the complex. Indeed, this brings information on which proteins can interact simultaneously with β-arrestins, or on the contrary, which partners are exclusive. We present here a method in two steps: protein-protein docking allows finding a limited number of peptides predicted to be involved in the interaction; and then experimental that might be used for validating the prediction. Keywords Protein complex β-arrestin partners Interface region Protein-protein docking Running title Interfaces between b-arrestins and their partners
Domaines
Biochimie [q-bio.BM]
Origine : Fichiers produits par l'(les) auteur(s)
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