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Chapitre D'ouvrage Année : 2019

Methods to Determine Interaction Interfaces Between β-Arrestins and Their Protein Partners

Résumé

β-arrestins are so-called hub proteins: they make complexes with many different partners, assembling functional complexes, and thereby fulfilling their biological function. The importance of this process in G protein-coupled receptor (GPCR) signalling has been fully demonstrated for many different receptors. For direct interactions, determining the interface regions, on β-arrestins and on the partners, is crucial for understanding the function of the complex. Indeed, this brings information on which proteins can interact simultaneously with β-arrestins, or on the contrary, which partners are exclusive. We present here a method in two steps: protein-protein docking allows finding a limited number of peptides predicted to be involved in the interaction; and then experimental that might be used for validating the prediction. Keywords Protein complex β-arrestin partners Interface region Protein-protein docking Running title Interfaces between b-arrestins and their partners
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Dates et versions

hal-02373790 , version 1 (21-11-2019)

Identifiants

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Thomas Bourquard, Astrid Musnier, Aurélie Trefier, Flavie Landomiel, Thomas Boulo, et al.. Methods to Determine Interaction Interfaces Between β-Arrestins and Their Protein Partners. Beta-arrestins- methods and protocols, 1957, , pp.177-194, 2019, Methods in Molecular Biology, ⟨10.1007/978-1-4939-9158-7_12⟩. ⟨hal-02373790⟩
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