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Article Dans Une Revue Nature Communications Année : 2019

ARTICLE RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis

Heike Lange
Simon y A Ndecky
  • Fonction : Auteur
Carlos Gomez-Diaz
  • Fonction : Auteur
Nicolas Butel
  • Fonction : Auteur
  • PersonId : 1018806
Julie Zumsteg
  • Fonction : Auteur
  • PersonId : 1054219
Christina Piermaria
  • Fonction : Auteur
Michael Christie
  • Fonction : Auteur
Ezgi S Karaaslan
  • Fonction : Auteur
Patricia L M Lang
  • Fonction : Auteur
Detlef Weigel
  • Fonction : Auteur
Hervé Vaucheret
Philippe Hammann
Dominique Gagliardi

Résumé

The RNA exosome is a key 3'−5' exoribonuclease with an evolutionarily conserved structure and function. Its cytosolic functions require the co-factors SKI7 and the Ski complex. Here we demonstrate by co-purification experiments that the ARM-repeat protein RESURRECTION1 (RST1) and RST1 INTERACTING PROTEIN (RIPR) connect the cytosolic Arabidopsis RNA exosome to the Ski complex. rst1 and ripr mutants accumulate RNA quality control siRNAs (rqc-siRNAs) produced by the post-transcriptional gene silencing (PTGS) machinery when mRNA degradation is compromised. The small RNA populations observed in rst1 and ripr mutants are also detected in mutants lacking the RRP45B/CER7 core exosome subunit. Thus, molecular and genetic evidence supports a physical and functional link between RST1, RIPR and the RNA exosome. Our data reveal the existence of additional cytosolic exosome co-factors besides the known Ski subunits. RST1 is not restricted to plants, as homologues with a similar domain architecture but unknown function exist in animals, including humans.
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hal-02378293 , version 1 (25-11-2019)

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Heike Lange, Simon y A Ndecky, Carlos Gomez-Diaz, David Pflieger, Nicolas Butel, et al.. ARTICLE RST1 and RIPR connect the cytosolic RNA exosome to the Ski complex in Arabidopsis. Nature Communications, 2019, 10, pp.1-12. ⟨10.1038/s41467-019-11807-4⟩. ⟨hal-02378293⟩
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