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Article Dans Une Revue International Journal of Molecular Sciences Année : 2019

Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology

Résumé

Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. A more recent class of split-FP variants, named « tripartite » split-FP, that rely on the self-assembly of three GFP fragments, is particularly well suited for the detection of protein-protein interactions. In this review, we describe the different steps and evolutions that have led to the diversification of superfolder and split-FP reporter systems, and we report an update of their applications in various areas of biology, from structural biology to cell biology.
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Dates et versions

hal-02378354 , version 1 (24-11-2020)

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Jean-Denis Pedelacq, Stéphanie Cabantous. Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology. International Journal of Molecular Sciences, 2019, 20 (14), pp.3479. ⟨10.3390/ijms20143479⟩. ⟨hal-02378354⟩
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