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Article Dans Une Revue Journal of Hepatology Année : 2018

The chaperone dynein LL1 mediates cytoplasmic transport of empty and mature hepatitis B virus capsids

Résumé

Background & Aims: Hepatitis B virus (HBV) has a DNA genome but replicates within the nucleus by reverse transcription of an RNA pregenome, which is converted to DNA in cytoplasmic capsids. Capsids in this compartment are correlated with inflammation and epitopes of the capsid protein core (Cp) are a major target for T cell-mediated immune responses. We investigated the mechanism of cytoplasmic capsid transport, which is important for infection but also for cytosolic capsid removal. Methods: We used virion-derived capsids containing mature rcDNA (matC) and empty capsids (empC). RNA-containing capsids (rnaC) were used as a control. The investigations comprised pull-down assays for identification of cellular interaction partners, immune fluorescence microscopy for their colocalization and electron microscopy after microinjection to determine their biological significance. Results: matC and empC underwent active transport through the cytoplasm towards the nucleus, while rnaC was poorly transported. We identified the dynein light chain LL1 as a functional interaction partner linking capsids to the dynein motor complex and showed that there is no compensatory transport pathway. Using capsid and dynein LL1 mutants we characterized the required domains on the capsid and LL1. Conclusions: This is the first investigation on the detailed molecular mechanism of how matC pass the cytoplasm upon infection and how empC can be actively removed from the cytoplasm into the nucleus. Considering that hepatocytes with cytoplasmic capsids are better recognized by the T cells, we hypothesize that targeting capsid DynLL1-interaction will not only block HBV infection but also stimulate elimination of infected cells
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hal-02406958 , version 1 (07-11-2023)

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Quentin Osseman, Lara Gallucci, Shelly Au, Christian Cazenave, Elodie Berdance, et al.. The chaperone dynein LL1 mediates cytoplasmic transport of empty and mature hepatitis B virus capsids. Journal of Hepatology, 2018, 68 (3), pp.441-448. ⟨10.1016/j.jhep.2017.10.032⟩. ⟨hal-02406958⟩
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