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Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2019

PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation

Frederique Tellier

Résumé

Lateral root organogenesis plays an essential role in elaborating plant root system architecture. In Arabidopsis, the AP2 family transcription factor PUCHI controls cell proliferation in lateral root primordia. To identify potential targets of PUCHI, we analyzed a time course transcriptomic dataset of lateral root formation. We report that multiple genes coding for very long chain fatty acid (VLCFA) biosynthesis enzymes are induced during lateral root development in a PUCHI-dependent manner. Significantly, several mutants perturbed in VLCFA biosynthesis show similar lateral root developmental defects as puchi-1. Moreover, puchi-1 roots display the same disorganized callus formation phenotype as VLCFA biosynthesis-deficient mutants when grown on auxin-rich callus-inducing medium. Lipidomic profiling of puchi-1 roots revealed reduced VLCFA content compared with WT. We conclude that PUCHI-regulated VLCFA biosynthesis is part of a pathway controlling cell proliferation during lateral root and callus formation.
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hal-02408581 , version 1 (25-10-2023)

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Paternité - Pas d'utilisation commerciale - Pas de modification

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Duy-Chi Trinh, Julien Lavenus, Tatsuaki Goh, Yohann Boutté, Quentin Drogue, et al.. PUCHI regulates very long chain fatty acid biosynthesis during lateral root and callus formation. Proceedings of the National Academy of Sciences of the United States of America, 2019, 116 (28), pp.14325-14330. ⟨10.1073/pnas.1906300116⟩. ⟨hal-02408581⟩
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