Genetic selection for active E.coIi amber tRNAASn exclusively led to glutamine inserting suppressors - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Nucleic Acids Research Année : 1995

Genetic selection for active E.coIi amber tRNAASn exclusively led to glutamine inserting suppressors

Résumé

Suppressor tRNAs are useful tools for determining identity elements which define recognition of tRNAs in vivo by their cognate aminoacyl-tRNA synthetases. This study was aimed at the isolation of active amber tRNAASn. Nineteen mutated tRNAASnfcuA having amber suppressor activity were selected by an in vivo genetic screen, and all exclusively inserted glutamine. From analysis of the different mutations it is concluded that glutamine accepting activity was obtained upon reducing the interaction strength between the first base pair of the tRNAASnfCuA by direct or indirect effects. Failure to isolate tRNAASnfcuA suppressors charged with asparagine as well as other evolutionary related amino acids is discussed.
Fichier principal
Vignette du fichier
1995-Martin.pdf (1.18 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02465316 , version 1 (03-02-2020)

Identifiants

Citer

Franck Martin, Gilbert Eriani, Joseph Reinbolt, Guy Dirheimer, Jean Gangloff. Genetic selection for active E.coIi amber tRNAASn exclusively led to glutamine inserting suppressors. Nucleic Acids Research, 1995, 23 (5), pp.779-784. ⟨10.1093/nar/23.5.779⟩. ⟨hal-02465316⟩

Collections

CNRS SITE-ALSACE
13 Consultations
41 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More