Class-A penicillin binding proteins do not contribute to cell shape but repair cell- wall defects - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue eLife Année : 2020

Class-A penicillin binding proteins do not contribute to cell shape but repair cell- wall defects

David Bikard

Résumé

Cell shape and cell-envelope integrity of bacteria are determined by the peptidoglycan cell wall. In rod-shaped Escherichia coli, two conserved sets of machinery are essential for cell-wall insertion in the cylindrical part of the cell: the Rod complex and the class-A penicillin-binding proteins (aPBPs). While the Rod complex governs rod-like cell shape, aPBP function is less well understood. aPBPs were previously hypothesized to either work in concert with the Rod complex or to independently repair cell-wall defects. First, we demonstrate through modulation of enzyme levels that aPBPs do not contribute to rod-like cell shape but are required for mechanical stability, supporting their independent activity. By combining measurements of cell-wall stiffness, cell-wall insertion, and PBP1b motion at the single-molecule level, we then present evidence that PBP1b, the major aPBP, contributes to cell-wall integrity by repairing cell wall defects.

Domaines

Bactériologie
Fichier principal
Vignette du fichier
elife-51998-v2.pdf (701.44 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02470461 , version 1 (07-02-2020)

Licence

Paternité

Identifiants

Citer

Antoine Vigouroux, Baptiste Cordier, Andrey Aristov, Laura Alvarez, Gizem Özbaykal, et al.. Class-A penicillin binding proteins do not contribute to cell shape but repair cell- wall defects. eLife, 2020, 9, pp.e51998. ⟨10.7554/eLife.51998⟩. ⟨hal-02470461⟩
78 Consultations
97 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More