A generic Microfluidic Approach for Deciphering Nanoscale biovesiclES propertieS - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Poster De Conférence Année : 2018

A generic Microfluidic Approach for Deciphering Nanoscale biovesiclES propertieS

Résumé

Extracellular vesicles (EVs) are vesicles shed by almost all cells with nanometric size and heterogeneous characteristics (biogenesis, shape…). EVs allow cell-to-cell communication and have a key role in lot of (patho)physiological reactions1. Since EVs cover a large size range (from 30 to 1000 nm) and originate from every cells, their characterization in their entirety represents a real challenge. Indeed, there is a lack of method enabling their isolation and analysis, in complex media, that impairs EVs to be qualified and used as biomarkers. Succeeding in EVs detection from their physiological environment and analyzing them in different subsets represent ambitious objective of many research groups. To reach this goal, one solution is to combine several technics and/or improve technical and preanalytical steps. We propose here to develop a microfluidic device, based on hydrodynamic filtration, also called deterministic lateral displacement : the aim of this system is to separate EVs to desired size if EVs are between sidewall and the cutoff radius (Rc), which are the maximum radius value allowing particle to pass in the lateral channel. A fluid flow, containing particles, is introduced into the main channel. In the cross section, only small portion of fluid pass in lateral channel: particles with radius smaller than Rc. This system will be coupled to a home-made gold biochip presenting microarrays enabling specific EVs subsets capture. Then, this lab-on-chip would make possible the recovery and analysis of EVs subsets at once.
Fichier principal
Vignette du fichier
poster Caparica_PILLEMONT.pdf (1.83 Mo) Télécharger le fichier
abstract_PILLEMONT_Caparica_2018_pdf.pdf (356.77 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02473108 , version 1 (10-02-2020)

Identifiants

  • HAL Id : hal-02473108 , version 1

Citer

Lyne Pillemont, Daniel Guneysu, Céline Elie-Caille, Wilfrid Boireau, Anne Marie Gué. A generic Microfluidic Approach for Deciphering Nanoscale biovesiclES propertieS. 3rd Caparica Christmas Conference on sample Treatment, Dec 2018, Caparica, Portugal. 2018. ⟨hal-02473108⟩
60 Consultations
11 Téléchargements

Partager

Gmail Facebook X LinkedIn More