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Article Dans Une Revue Optics Express Année : 2020

High-speed chemical imaging of dynamic and histological samples with stimulated Raman micro-spectroscopy

Xavier Audier
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Nicolas Forget
Herve Rigneault

Résumé

We report a shot noise limited high-speed stimulated Raman microscopy platform allowing to acquire molecular vibrational spectra over 200 cm −1 in 12 µs at a scan rate of 40kHz. Using spectral focusing together with optimized acousto-optics programmable dispersive filters, the designed low noise imaging platform performs chemical imaging of dynamical processes such as Mannitol crystal hydration and reaches a signal to noise ratio sufficient to perform label free histological imaging on frozen human colon tissue slides.
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Dates et versions

hal-02567583 , version 1 (07-05-2020)

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Xavier Audier, Nicolas Forget, Herve Rigneault. High-speed chemical imaging of dynamic and histological samples with stimulated Raman micro-spectroscopy. Optics Express, 2020, 28 (10), pp.15505. ⟨10.1364/OE.390850⟩. ⟨hal-02567583⟩
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