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Article Dans Une Revue Journal of Biological Chemistry Année : 2015

Experimental evidence for a hydride transfer mechanism in plant glycolate oxidase catalysis

Caroline Mauve
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Edouard Boex-Fontvieille
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Valérie Flesch
  • Fonction : Auteur
Mathieu Jossier
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Guillaume Tcherkez
Michael Hodges

Résumé

In plants, glycolate oxidase is involved in the photorespiratory cycle, one of the major fluxes at the global scale. To clarify both the nature of the mechanism and possible differences in glycolate oxidase enzyme chemistry from C3 and C4 plant species, we analyzed kinetic parameters of purified recombinant C3 (Arabidopsis thaliana) and C4 (Zea mays) plant enzymes and compared isotope effects using natural and deuterated glycolate in either natural or deuterated solvent. The (12)C/(13)C isotope effect was also investigated for each plant glycolate oxidase protein by measuring the (13)C natural abundance in glycolate using natural or deuterated glycolate as a substrate. Our results suggest that several elemental steps were associated with an hydrogen/deuterium isotope effect and that glycolate α-deprotonation itself was only partially rate-limiting. Calculations of commitment factors from observed kinetic isotope effect values support a hydride transfer mechanism. No significant differences were seen between C3 and C4 enzymes.
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hal-02634445 , version 1 (27-05-2020)

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Younes Dellero, Caroline Mauve, Edouard Boex-Fontvieille, Valérie Flesch, Mathieu Jossier, et al.. Experimental evidence for a hydride transfer mechanism in plant glycolate oxidase catalysis. Journal of Biological Chemistry, 2015, 290 (3), pp.1689-1698. ⟨10.1074/jbc.M114.618629⟩. ⟨hal-02634445⟩
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