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Article Dans Une Revue Plant Physiology Année : 2016

Revisiting plant plasma membrane lipids in tobacco: a focus on sphingolipids

Laetitia Fouillen
Emmanuelle Bayer
Françoise Simon Plas

Résumé

The lipid composition of plasma membrane (PM) and the corresponding detergent-insoluble membrane (DIM) fraction were analyzed with a specific focus on highly polar sphingolipids, so-called glycosyl inositol phosphorylceramides (GIPCs). Using tobacco (Nicotiana tabacum) 'Bright Yellow 2' cell suspension and leaves, evidence is provided that GIPCs represent up to 40 mol % of the PM lipids. Comparative analysis of DIMs with the PM showed an enrichment of 2-hydroxylated very-long-chain fatty acid-containing GIPCs and polyglycosylated GIPCs in the DIMs. Purified antibodies raised against these GIPCs were further used for immunogold-electron microscopy strategy, revealing the distribution of polyglycosylated GIPCs in domains of 35 +/- 7 nm in the plane of the PM. Biophysical studies also showed strong interactions between GIPCs and sterols and suggested a role for very-long-chain fatty acids in the interdigitation between the two PM-composing monolayers. The ins and outs of lipid asymmetry, raft formation, and interdigitation in plant membrane biology are finally discussed.

Dates et versions

hal-02637209 , version 1 (27-05-2020)

Identifiants

Citer

Jean-Luc Cacas, Corinne Bure, Kévin Grosjean, Patricia Gerbeau Pissot, Jeannine Lherminier, et al.. Revisiting plant plasma membrane lipids in tobacco: a focus on sphingolipids. Plant Physiology, 2016, 170 (1), pp.367-384. ⟨10.1104/pp.15.00564⟩. ⟨hal-02637209⟩
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