A protein kinase binds the C-terminal domain of the readthrough protein of Turnip yellows virus and regulates virus accumulation - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Virology Année : 2015

A protein kinase binds the C-terminal domain of the readthrough protein of Turnip yellows virus and regulates virus accumulation

Résumé

Turnip yellows virus (TuYV), a phloem-limited virus, encodes a 74 kDa protein known as the readthrough protein (RT) involved in virus movement. We show here that a TuYV mutant deleted of the C-terminal part of the RT protein (TuYV-ΔRTCter) was affected in long-distance trafficking in a host-specific manner. By using the C-terminal domain of the RT protein as a bait in a yeast two-hybrid screen of a phloem cDNA library from Arabidopsis thaliana we identified the calcineurin B-like protein-interacting protein kinase-7 (AtCIPK7). Transient expression of a GFP:CIPK7 fusion protein in virus-inoculated Nicotiana benthamiana leaves led to local increase of wild-type TuYV accumulation, but not that of TuYV-ΔRTCter. Surprisingly, elevated virus titer in inoculated leaves did not result in higher TuYV accumulation in systemic leaves, which indicates that virus long-distance movement was not affected. Since GFP:CIPK7 was localized in or near plasmodesmata, CIPK7 could negatively regulate TuYV export from infected cells.
Fichier principal
Vignette du fichier
Rodriguez, Boissinot et al Virology 2015_1.pdf (920.18 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02640267 , version 1 (28-05-2020)

Identifiants

Citer

Caren Rodriguez-Medina, Sylvaine Boissinot, Sophie Chapuis, Dalya Gereige, Maryam Rastegar, et al.. A protein kinase binds the C-terminal domain of the readthrough protein of Turnip yellows virus and regulates virus accumulation. Virology, 2015, 486, pp.44-53. ⟨10.1016/j.virol.2015.08.031⟩. ⟨hal-02640267⟩
50 Consultations
64 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More