Specific membrane lipid composition is important for Plasmodesmata function in Arabidopsis - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue The Plant cell Année : 2015

Specific membrane lipid composition is important for Plasmodesmata function in Arabidopsis

Stéphane Claverol

Résumé

Plasmodesmata (PD) are nano-sized membrane-lined channels controlling intercellular communication in plants. Although progress has been made in identifying PD proteins, the role played by major membrane constituents, such as the lipids, in defining specialized membrane domains in PD remains unknown. Through a rigorous isolation of "native" PD membrane fractions and comparative mass spectrometry-based analysis, we demonstrate that lipids are laterally segregated along the plasma membrane (PM) at the PD cell-to-cell junction in Arabidopsis thaliana. Remarkably, our results show that PD membranes display enrichment in sterols and sphingolipids with very long chain saturated fatty acids when compared with the bulk of the PM. Intriguingly, this lipid profile is reminiscent of detergent-insoluble membrane microdomains, although our approach is valuably detergent-free. Modulation of the overall sterol composition of young dividing cells reversibly impaired the PD localization of the glycosylphosphatidylinositol-anchored proteins Plasmodesmata Callose Binding 1 and the beta-1,3-glucanase PdBG2 and altered callose-mediated PD permeability. Altogether, this study not only provides a comprehensive analysis of the lipid constituents of PD but also identifies a role for sterols in modulating cell-to-cell connectivity, possibly by establishing and maintaining the positional specificity of callose-modifying glycosylphosphatidylinositol proteins at PD. Our work emphasizes the importance of lipids in defining PD membranes.

Dates et versions

hal-02641388 , version 1 (28-05-2020)

Identifiants

Citer

Magali S. Grison, Lysiane Brocard, Laetitia Fouillen, William Nicolas, Vera Wewer, et al.. Specific membrane lipid composition is important for Plasmodesmata function in Arabidopsis. The Plant cell, 2015, 27 (4), pp.1228-1250. ⟨10.1105/tpc.114.135731⟩. ⟨hal-02641388⟩
36 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More