RNA captor: a tool for RNA characterization - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2011

RNA captor: a tool for RNA characterization

Christian Clepet
  • Fonction : Auteur
  • PersonId : 748882
  • IdHAL : clepet

Résumé

Background: In the genome era, characterizing the structure and the function of RNA molecules remains a major challenge. Alternative transcripts and non-protein-coding genes are poorly recognized by the current genome-annotation algorithms and efficient tools are needed to isolate the less-abundant or stable RNAs. Results: A universal RNA-tagging method using the T4 RNA ligase 2 and special adapters is reported. Based on this system, protocols for RACE PCR and full-length cDNA library construction have been developed. The RNA tagging conditions were thoroughly optimized and compared to previous methods by using a biochemical oligonucleotide tagging assay and RACE PCRs on a range of transcripts. In addition, two large-scale full-length cDNA inventories relying on this method are presented. Conclusion: The RNA Captor is a straightforward and accessible protocol. The sensitivity of this approach was shown to be higher compared to previous methods, and applicable on messenger RNAs, non-protein-coding RNAs, transcription-start sites and microRNA-directed cleavage sites of transcripts. This strategy could also be used to study other classes of RNA and in deep sequencing experiments.
Fichier principal
Vignette du fichier
2011_Clepet_Plosone_1.pdf (691.48 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02645463 , version 1 (29-05-2020)

Identifiants

Citer

Christian Clepet. RNA captor: a tool for RNA characterization. PLoS ONE, 2011, 6 (4), pp.e18445. ⟨10.1371/journal.pone.0018445⟩. ⟨hal-02645463⟩
18 Consultations
28 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More