Study of Helicobacter pullorum proinflammatory properties on human epithelial cells in vitro - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Gut Année : 2009

Study of Helicobacter pullorum proinflammatory properties on human epithelial cells in vitro

Résumé

Background and aims: Helicobacter pullorum is an enterohepatic Helicobacter species of avian origin detected in patients with acute diarrhoea and inflammatory bowel disease. The aim of the present study was to determine whether H pullorum exerts a direct effect on human intestinal epithelial cells in vitro and to characterise the bacterial mechanisms and the signalling pathways involved. Materials and methods: The proinflammatory properties of H pullorum from human and avian origins were measured on human gastric (AGS) and intestinal (CaCo-2 and HT-29) epithelial cell lines after co-culture with different H pullorum strains, and the extent of nuclear factor-κB (NF-κB) involvement was determined. Results: All of the H pullorum strains tested stimulated interleukin 8 (IL8) secretion by the three cell lines. Similar results were obtained with heat-killed H pullorum. Incubation of cells with filtered H pullorum culture supernatants did not stimulate IL8 secretion. The same observation was made when bacterial adherence was inhibited by Transwell inserts. H pullorum induced NF-κB activation and rapid nuclear translocation as demonstrated by immunofluorescent staining and cellular fractionation. NF-κB involvement was confirmed by using the specific inhibitor SN50 and small interfering RNA (siRNA) which abolished H pullorum-induced IL8 production. Conclusions: H pullorum strains stimulate IL8 secretion by human gastric and intestinal epithelial cell lines. This effect requires bacterial adherence and probably lipopolysaccharides, and is mediated by NF-κB signalling. The present study strengthens the argument that H pullorum is a potent human pathogen and highlights its putative role in acute and chronic digestive diseases such as inflammatory bowel disease.

Dates et versions

hal-02662939 , version 1 (31-05-2020)

Identifiants

Citer

C. Varon, A. Duriez, Philippe Lehours, A. Ménard, Sophie Layé, et al.. Study of Helicobacter pullorum proinflammatory properties on human epithelial cells in vitro. Gut, 2009, 58 (5), pp.629-635. ⟨10.1136/gut.2007.144501⟩. ⟨hal-02662939⟩
15 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More