Universal DNA-Based methods for Assessing the diet of grazing livestock and wildlife from feces - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Agricultural and Food Chemistry Année : 2009

Universal DNA-Based methods for Assessing the diet of grazing livestock and wildlife from feces

Résumé

Because of the demand for controlling livestock diets, two methods that characterize the DNA of plants present in feces were developed. After DNA extraction from fecal samples, a short fragment of the chloroplastic trnL intron was amplified by PCR using a universal primer pair for plants. The first method generates a signature that is the electrophoretic migration pattern of the PCR product. The second method consists of sequencing several hundred DNA fragments from the PCR product through pyrosequencing. These methods were validated with a blind analysis of feces from concentrate- and pasture-fed lambs. The signature method allowed differentiation of the two diets and confirmed the presence of concentrate in one of them. The pyrosequencing method allowed the identification of up to 25 taxa in a diet. These methods are complementary to the chemical methods already used. They could be applied to the control of diets and the study of food preferences.
Fichier non déposé

Dates et versions

hal-02666465 , version 1 (31-05-2020)

Identifiants

Citer

Anthony Pegard, Christian Miquel, Alice Valentini, Eric Coissac, Frédéric F. Bouvier, et al.. Universal DNA-Based methods for Assessing the diet of grazing livestock and wildlife from feces. Journal of Agricultural and Food Chemistry, 2009, 57 (13), pp.5700-5706. ⟨10.1021/jf803680c⟩. ⟨hal-02666465⟩
35 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More