Involvement of DnaE, the second replicative DNA polymerase from Bacillus subtilis, in DNA mutagenesis. - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 2004

Involvement of DnaE, the second replicative DNA polymerase from Bacillus subtilis, in DNA mutagenesis.

Résumé

In a large group of organisms including low G + C bacteria and eukaryotic cells, DNA synthesis at the replication fork strictly requires two distinct replicative DNA polymerases. These are designated pol C and DnaE in Bacillus subtilis. We recently proposed that DnaE might be preferentially involved in lagging strand synthesis, whereas pol C would mainly carry out leading strand synthesis. The biochemical analysis of DnaE reported here is consistent with its postulated function, as it is a highly potent enzyme, replicating as fast as 240 nucleotides/s, and stalling for more than 30 s when encountering annealed 5'-DNA end. DnaE is devoid of 3' --> 5'-proofreading exonuclease activity and has a low processivity (1-75 nucleotides), suggesting that it requires additional factors to fulfill its role in replication. Interestingly, we found that (i) DnaE is SOS-inducible; (ii) variation in DnaE or pol C concentration has no effect on spontaneous mutagenesis; (iii) depletion of pol C or DnaE prevents UV-induced mutagenesis; and (iv) purified DnaE has a rather relaxed active site as it can bypass lesions that generally block other replicative polymerases. These results suggest that DnaE and possibly pol C have a function in DNA repair/mutagenesis, in addition to their role in DNA replication.
Fichier principal
Vignette du fichier
2004_Le Chatelier_Journal of Biological Chemistry_1.pdf (498.13 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02681922 , version 1 (01-06-2020)

Licence

Copyright (Tous droits réservés)

Identifiants

Citer

Emmanuelle E. Le Chatelier, Olivier Bécherel, Emmanuelle d'Alençon, Danielle Canceill, S. Dusko Ehrlich, et al.. Involvement of DnaE, the second replicative DNA polymerase from Bacillus subtilis, in DNA mutagenesis.. Journal of Biological Chemistry, 2004, 279 (3), pp.1757-1767. ⟨10.1074/jbc.M310719200⟩. ⟨hal-02681922⟩
15 Consultations
32 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More