Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 2000

Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family

Résumé

Grb7 is a member of a family of molecular adapters which are able to contribute positively but also negatively to signal transduction and whose precise roles remain obscure. Rnd1 is a member of the Rho family, but, as opposed to usual GTPases, it is constitutively bound to GTP. We show here that Rnd1 and Grb7 interact, in two-hybrid assays, in vitro, and in pull-down experiments performed with SK-BR3, a breast cancer cell line that overexpresses Grb7. This interaction involves switch II loop of Rnd1, a region crucial for guanine nucleotide exchange in all GTPases, and a Grb7 SH2 domain, a region crucial for Grb7 interaction with several activated receptors. The contribution of the interaction between Rnd1 and Grb7 to their respective functions and properties is discussed.

Mots clés

Domaines

Autre [q-bio.OT]

Dates et versions

hal-02695354 , version 1 (01-06-2020)

Identifiants

Citer

Béatrice Vayssière, Gérard Zalcman, Yannick Mahe, Gladys Mirey, Tanja Ligensa, et al.. Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family. FEBS Letters, 2000, 467 (1), pp.91-96. ⟨10.1016/S0014-5793(99)01530-6⟩. ⟨hal-02695354⟩
7 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More