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Article Dans Une Revue European Journal of Biochemistry Année : 1993

Determinants of the brain-specific expression of the rat aldolase C gene: ex vivo and in vivo analysis

Muriel M. Thomas
Iman Makeh
  • Fonction : Auteur
Pascale Briand
  • Fonction : Auteur
Axel Kahn
  • Fonction : Auteur
Henriette Skala
  • Fonction : Auteur

Résumé

A 115-bp promoter fragment of the aldolase C gene is sufficient for conferring neural cell specificity on a reporter gene, in cultured PC12 cells and in transgenic mice. In vitro DNase I protection experiments detected two footprints on the promoter, termed boxes A/A', and B. The 5' A/A' box contains overlapping Sp1 and Krox20/Krox24 binding sites; it binds Sp1 in fibroblasts (box A') and a different complex in brain (box A). Any deletion or mutation of this box that impairs protein recognition also suppresses promoter activity. The replacement of box A/A' by a Sp1 consensus binding site results in the loss of the brain specificity of expression in transgenic mice. Further 3', box B is composed of a 5' direct repeat and a 3' GC box consisting of overlapping Sp1 and Krox20/Krox24 binding sites. Mutation of the direct repeat subregion appears to be more deleterious for the promoter activity than mutation of the G+C-rich subregion.
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Dates et versions

hal-02713367 , version 1 (01-06-2020)

Identifiants

  • HAL Id : hal-02713367 , version 1
  • PRODINRA : 342468
  • PUBMED : 8243460
  • WOS : A1993MH03300014

Citer

Muriel M. Thomas, Iman Makeh, Pascale Briand, Axel Kahn, Henriette Skala. Determinants of the brain-specific expression of the rat aldolase C gene: ex vivo and in vivo analysis. European Journal of Biochemistry, 1993, 218 (1), pp.143-151. ⟨hal-02713367⟩
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